Natural Hybrids of Phytophthora nicotianae and Phytophthora cactorum Demonstrated by Isozyme Analysis and Random Amplified Polymorphic DNA.
Identifieur interne : 002978 ( Main/Exploration ); précédent : 002977; suivant : 002979Natural Hybrids of Phytophthora nicotianae and Phytophthora cactorum Demonstrated by Isozyme Analysis and Random Amplified Polymorphic DNA.
Auteurs : W A Man In T Veld ; W J Veenbaas-Rijks ; E. Ilieva ; A W De Cock ; P J Bonants ; R. PietersSource :
- Phytopathology [ 0031-949X ] ; 1998.
Abstract
ABSTRACT Three similar isolates of Phytophthora (Phytophthora sp-h) were obtained from diseased Spathiphyllum and Primula plants. Cultural characteristics did not fit any known description of Phytophthora species. The Phytophthora sp-h isolates are papillate, are homothallic, possess 80 to 86% amphigynous antheridia, and have a maximum temperature for growth of 36.5 degrees C. Isozyme analysis of the Phytophthora sp-h isolates revealed a three-banded pattern with malic enzyme and a three-banded pattern with malate dehydrogenase on the second putative locus. The fastest band at both enzyme loci comigrated with the single P. nicotianae band, the slowest band comigrated with the single P. cactorum (and also P. pseudotsugae) band, and one band in between was concluded to represent the heterodimeric isozyme. The random amplified polymorphic DNA patterns of the Phytophthora sp-h isolates almost exclusively consisted of bands that were also present in either P. nicotianae or P. cactorum. Southern hybridization showed that bands specific for P. nicotianae were present as comigrating bands in the Phytophthora sp-h isolates. The same was found for species-specific bands of P. cactorum. It is concluded that the three Phytophthora sp-h isolates represent interspecific hybrids, P. nicotianae being the one parent and P. cactorum the other. Analysis of mito-chondrial DNA with restriction enzymes revealed banding patterns in all the Phytophthora sp-h isolates identical with those of P. nicotianae, confirming that indeed P. nicotianae was one of the parents.
DOI: 10.1094/PHYTO.1998.88.9.922
PubMed: 18944870
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Natural Hybrids of Phytophthora nicotianae and Phytophthora cactorum Demonstrated by Isozyme Analysis and Random Amplified Polymorphic DNA.</title><author><name sortKey="Man In T Veld, W A" sort="Man In T Veld, W A" uniqKey="Man In T Veld W" first="W A" last="Man In T Veld">W A Man In T Veld</name></author><author><name sortKey="Veenbaas Rijks, W J" sort="Veenbaas Rijks, W J" uniqKey="Veenbaas Rijks W" first="W J" last="Veenbaas-Rijks">W J Veenbaas-Rijks</name></author><author><name sortKey="Ilieva, E" sort="Ilieva, E" uniqKey="Ilieva E" first="E" last="Ilieva">E. Ilieva</name></author><author><name sortKey="De Cock, A W" sort="De Cock, A W" uniqKey="De Cock A" first="A W" last="De Cock">A W De Cock</name></author><author><name sortKey="Bonants, P J" sort="Bonants, P J" uniqKey="Bonants P" first="P J" last="Bonants">P J Bonants</name></author><author><name sortKey="Pieters, R" sort="Pieters, R" uniqKey="Pieters R" first="R" last="Pieters">R. Pieters</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:18944870</idno><idno type="pmid">18944870</idno><idno type="doi">10.1094/PHYTO.1998.88.9.922</idno><idno type="wicri:Area/Main/Corpus">001C13</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001C13</idno><idno type="wicri:Area/Main/Curation">001C13</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001C13</idno><idno type="wicri:Area/Main/Exploration">001C13</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Natural Hybrids of Phytophthora nicotianae and Phytophthora cactorum Demonstrated by Isozyme Analysis and Random Amplified Polymorphic DNA.</title><author><name sortKey="Man In T Veld, W A" sort="Man In T Veld, W A" uniqKey="Man In T Veld W" first="W A" last="Man In T Veld">W A Man In T Veld</name></author><author><name sortKey="Veenbaas Rijks, W J" sort="Veenbaas Rijks, W J" uniqKey="Veenbaas Rijks W" first="W J" last="Veenbaas-Rijks">W J Veenbaas-Rijks</name></author><author><name sortKey="Ilieva, E" sort="Ilieva, E" uniqKey="Ilieva E" first="E" last="Ilieva">E. Ilieva</name></author><author><name sortKey="De Cock, A W" sort="De Cock, A W" uniqKey="De Cock A" first="A W" last="De Cock">A W De Cock</name></author><author><name sortKey="Bonants, P J" sort="Bonants, P J" uniqKey="Bonants P" first="P J" last="Bonants">P J Bonants</name></author><author><name sortKey="Pieters, R" sort="Pieters, R" uniqKey="Pieters R" first="R" last="Pieters">R. Pieters</name></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">ABSTRACT Three similar isolates of Phytophthora (Phytophthora sp-h) were obtained from diseased Spathiphyllum and Primula plants. Cultural characteristics did not fit any known description of Phytophthora species. The Phytophthora sp-h isolates are papillate, are homothallic, possess 80 to 86% amphigynous antheridia, and have a maximum temperature for growth of 36.5 degrees C. Isozyme analysis of the Phytophthora sp-h isolates revealed a three-banded pattern with malic enzyme and a three-banded pattern with malate dehydrogenase on the second putative locus. The fastest band at both enzyme loci comigrated with the single P. nicotianae band, the slowest band comigrated with the single P. cactorum (and also P. pseudotsugae) band, and one band in between was concluded to represent the heterodimeric isozyme. The random amplified polymorphic DNA patterns of the Phytophthora sp-h isolates almost exclusively consisted of bands that were also present in either P. nicotianae or P. cactorum. Southern hybridization showed that bands specific for P. nicotianae were present as comigrating bands in the Phytophthora sp-h isolates. The same was found for species-specific bands of P. cactorum. It is concluded that the three Phytophthora sp-h isolates represent interspecific hybrids, P. nicotianae being the one parent and P. cactorum the other. Analysis of mito-chondrial DNA with restriction enzymes revealed banding patterns in all the Phytophthora sp-h isolates identical with those of P. nicotianae, confirming that indeed P. nicotianae was one of the parents.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">18944870</PMID><DateCompleted><Year>2012</Year><Month>10</Month><Day>02</Day></DateCompleted><DateRevised><Year>2008</Year><Month>10</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><Volume>88</Volume><Issue>9</Issue><PubDate><Year>1998</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Natural Hybrids of Phytophthora nicotianae and Phytophthora cactorum Demonstrated by Isozyme Analysis and Random Amplified Polymorphic DNA.</ArticleTitle><Pagination><MedlinePgn>922-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO.1998.88.9.922</ELocationID><Abstract><AbstractText>ABSTRACT Three similar isolates of Phytophthora (Phytophthora sp-h) were obtained from diseased Spathiphyllum and Primula plants. Cultural characteristics did not fit any known description of Phytophthora species. The Phytophthora sp-h isolates are papillate, are homothallic, possess 80 to 86% amphigynous antheridia, and have a maximum temperature for growth of 36.5 degrees C. Isozyme analysis of the Phytophthora sp-h isolates revealed a three-banded pattern with malic enzyme and a three-banded pattern with malate dehydrogenase on the second putative locus. The fastest band at both enzyme loci comigrated with the single P. nicotianae band, the slowest band comigrated with the single P. cactorum (and also P. pseudotsugae) band, and one band in between was concluded to represent the heterodimeric isozyme. The random amplified polymorphic DNA patterns of the Phytophthora sp-h isolates almost exclusively consisted of bands that were also present in either P. nicotianae or P. cactorum. Southern hybridization showed that bands specific for P. nicotianae were present as comigrating bands in the Phytophthora sp-h isolates. The same was found for species-specific bands of P. cactorum. It is concluded that the three Phytophthora sp-h isolates represent interspecific hybrids, P. nicotianae being the one parent and P. cactorum the other. Analysis of mito-chondrial DNA with restriction enzymes revealed banding patterns in all the Phytophthora sp-h isolates identical with those of P. nicotianae, confirming that indeed P. nicotianae was one of the parents.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Man In 't Veld</LastName><ForeName>W A</ForeName><Initials>WA</Initials></Author><Author ValidYN="Y"><LastName>Veenbaas-Rijks</LastName><ForeName>W J</ForeName><Initials>WJ</Initials></Author><Author ValidYN="Y"><LastName>Ilieva</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>de Cock</LastName><ForeName>A W</ForeName><Initials>AW</Initials></Author><Author ValidYN="Y"><LastName>Bonants</LastName><ForeName>P J</ForeName><Initials>PJ</Initials></Author><Author ValidYN="Y"><LastName>Pieters</LastName><ForeName>R</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>10</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>10</Month><Day>24</Day><Hour>9</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18944870</ArticleId><ArticleId IdType="doi">10.1094/PHYTO.1998.88.9.922</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Bonants, P J" sort="Bonants, P J" uniqKey="Bonants P" first="P J" last="Bonants">P J Bonants</name><name sortKey="De Cock, A W" sort="De Cock, A W" uniqKey="De Cock A" first="A W" last="De Cock">A W De Cock</name><name sortKey="Ilieva, E" sort="Ilieva, E" uniqKey="Ilieva E" first="E" last="Ilieva">E. Ilieva</name><name sortKey="Man In T Veld, W A" sort="Man In T Veld, W A" uniqKey="Man In T Veld W" first="W A" last="Man In T Veld">W A Man In T Veld</name><name sortKey="Pieters, R" sort="Pieters, R" uniqKey="Pieters R" first="R" last="Pieters">R. Pieters</name><name sortKey="Veenbaas Rijks, W J" sort="Veenbaas Rijks, W J" uniqKey="Veenbaas Rijks W" first="W J" last="Veenbaas-Rijks">W J Veenbaas-Rijks</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002978 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002978 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:18944870
|texte= Natural Hybrids of Phytophthora nicotianae and Phytophthora cactorum Demonstrated by Isozyme Analysis and Random Amplified Polymorphic DNA.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18944870" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |